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Yes, several things hint to this. For one, Breen was in charge of the Test at Black Mesa that started everything and insisted that it be carried out beyond the normal powers settings for such a test. Furthermore, he practically comes right out and mentions the G-man when he asks Gordon if he knew his contract was open to the highest bidder. Also, the G-man seems to refer to Breen as one of the naysayers who claimed Alyx was of no use to anyone, especially since a picture of Bren flashes on the screen at that moment.

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